Prevalence of suicide ideation among middle school students in China: a systematic analysis of studies between 2000 and 2012 / 中华流行病学杂志

<p><b>OBJECTIVE</b>To analyze the prevalence and differences between gender and grades of suicide ideation among middle school students in China from 2000 to 2012 so as to provide basis for suicide prevention among middle school students.</p><p><b>METHODS</b>Electronic search strategy was carried out, using PubMed, Wanfang Database, VIP Database, China National Knowledge Infrastructure (CNKI) and CBM to collect data on suicide ideation among middle school students. Fixed effects model or random effects model was employed according to statistical tests for the homogeneity. Publication bias was assessed by rank correlation test.</p><p><b>RESULTS</b>40 papers were included for meta-analysis, with a total sample size of 320 375. The combined prevalence of suicide ideation was 17.99% (95%CI: 16.59% - 19.49%). Prevalence rates of suicide ideation were stratified by factors as gender and grade at school. Pooled prevalence rates on suicide ideation were as follows: 14.71% (95%CI: 13.42% - 16.11%) and 19.92% (95%CI: 19.30% - 21.64%), P < 0.05 for boys and girls;16.94% (95%CI: 15.35% - 18.66%) and 19.01% (95%CI: 17.23% - 20.93%), P < 0.05 for senior or junior high school students, respectively.</p><p><b>CONCLUSION</b>There were differences in the prevalence of suicide ideation among middle school students between genders and grades in China.</p>

Clinical analysis of autoimmune pancreatitis: a single center study of 14 consecutive cases / 中华外科杂志

<p><b>OBJECTIVE</b>To investigate the characteristic findings of autoimmune pancreatitis (AIP) to increase the recognition of AIP.</p><p><b>METHODS</b>From February 2002 to April 2008, a total of 14 cases of AIP were reviewed by clinical, imaging, serologic, histopathologic features and treatment response. There were 13 male and 1 female, with a mean age of 53 years. The main clinical manifestations included progressive obstructive jaundice in 11 cases, upper abdomen pain in 3 cases.</p><p><b>RESULTS</b>Diffuse enlargement of pancreas and diffuse narrowing of the main pancreatic duct (MPD) were observed in 11 cases, while 3 patients showed localized pancreatic head enlargement and focal narrowing of the MPD. Distal common bile duct stenosis was found in all cases. Increased expression of serum immunoglobulin G was found in 7 patients. Autoantibody test was positive in 5 of 12 patients. Nine of 14 patients with AIP had extrapancreatic organ involvement. Massive lymphocytes and plasma cells infiltration in pancreatic tissues were showed on pathology, as well as parenchymal fibrosis. Seven earlier patients were treated initially with surgical laparotomy or resection for suspected malignancy. Steroid therapy was given to the other patients and was responsive. There were 4 recurrences after initial treatment.</p><p><b>CONCLUSION</b>AIP should be a differential diagnosis in pancreatic head mass in order to avoid unnecessary resection.</p>

The effect of iNOS gene expression inhibited by RNA inference on the pancreas islet apoptosis and function in rats / 中华外科杂志

<p><b>OBJECTIVE</b>To investigate the effect of iNOS gene on cell apoptosis and insulin secretion of pancreas islet in rats by RNA inference (RNAi).</p><p><b>METHODS</b>Islets obtained from thirty Wistar rats were randomly divided into five groups, and siRNA oligo was purchased from Genepharma in Shanghai. The cultured islets were transfected with iNOS siRNA, and then were divided into five groups. Islet cultured only was taken as blank control group, and cultured with TNF-alpha + IL-1 beta as cytokine group. Islet transfected with negative or iNOS siRNA were taken as negative transfection control group or RNAi group, while that transfected with iNOS siRNA and cultured with TNF-alpha + IL-1 beta as RNAi + cytokine group. Expression of iNOS mRNA was evaluated by RT-PCR and iNOS protein was evaluated by Western blot to detect the effect of RNAi. The expression of apoptosis correlated gene, Bax, Fas were analyzed, and the apoptotic cells were identified by TUNEL method meanwhile. Insulin secretion index assay the function of the islets.</p><p><b>RESULTS</b>500 - 600 IEQ islets could be extracted from every rat. RNAi attenuated the expression of iNOS and restrained the synthesis of iNOS protein.With treatment of cytokines IL-1 beta and TNF-alpha, the level of iNOS increased remarkably, the expression of Bax and Fas ascended distinctly, and insulin secretion index decreased strikingly. While, the expression of apoptosis gene and amount of apoptotic cells descended in group of RNAi + cytokine, and insulin secretion index were satisfying.</p><p><b>CONCLUSION</b>The apoptosis from cytokines to islets mediated by iNOS could be suppressed by RNAi, which leaded to favorable function and survival of islets.</p>

Effect of Composite Radix Sophora Flavescentis Injection on Immunological Function in Patients with Lung Cancer / 中国药房

OBJECTIVE:To test and verify the function of Composite Radix Sophora Flavescentis injection in immunologic enhancement.METHODS:A total of 121 patients with lung cancer were randomly assigned to 2 groups:the treatment group(n=61)received common chemotherapy for 6wk plan in combination with Composite Radix Sophora Flavescentis injection(20mL,iv gtt)qd for the first 10 to 14 days,while the control group(n=60)received common chemotherapy alone for 6wk.The indexes of immunity in both groups were detected before and after treatment.RESULTS:The index of immunity in the treatment group increased significantly as compared with before treatment(P

Adenosine protects cardiomyocytes from hypoxia/reoxygenation injury / 生理学报

The aim of this study was to investigate the protective effect of adenosine (ADO) on cardiomyocytes following hypoxia/reoxygenation (H/R) and its molecular mechanism. Primary cultured cardiomyocytes of neonatal rats were divided into two groups, namely H/R (control) and ADO (1.0 micromol/L) groups. The morphologic changes in cardiomyocytes were observed under an inverted phase-contrast microscope. The following parameters of the two groups were determined: lactate dehydrogenase (LDH) activity, intracellular calcium concentration and malondialdehyde (MDA) content. Tumor necrotic factor (TNF-alpha) assay was performed using an ELISA kit and NF-kappaB in the nucleus was analyzed by electrophoretic mobility shift assay (EMSA). The results are as follows: (1) after H/R injury, cardiomyocytes contracted, tending to get round in shape and its pseudopods decreased, while marked morphological changes were not observed in ADO group; (2) LDH leakage maintained at a lower level in ADO group than that in the control group during H/R (both P<0.01); (3) ADO significantly reduced the concentration of calcium in cells and prevented calcium overload during H/R (both P<0.01); (4) ADO markedly reduced the content of MDA during H/R (both P<0.01); (5) ADO inhibited the production of TNF-alpha during H/R (both P<0.01); and (6) ADO down-regulated NF-kappaB binding activity of cardiomyocytes during H/R (both P<0.01) The results suggest that (1) exogenous ADO attenuates H/R injury of cultured cardiomyocytes; (2) exogenous ADO inhibits the production of TNF-alpha after H/R injury; (3) exogenous ADO prevents the activation of NF-kappaB, which may be the molecular mechanism of down-regulation of TNF-alpha expression.

Study on drug-resistance of Mycobacterium tuberculosis isolated from coal workers with silico-tuberculosis / 中华劳动卫生职业病杂志

<p><b>OBJECTIVE</b>To study the gene mutation and streptomycin, isoniazid or rifampicin resistance of Mycobacterium isolated from silico-tuberculosis patient's sputum so as to find a more effective therapy for this disease.</p><p><b>METHODS</b>Mycobacteria tuberculosis were separated from 96 coal worker with silico-tuberculosis firstly. Then rpsL, KatG and rpoB fragments of genome were copied with PCR and compared their SSCP profiles with standard strains.</p><p><b>RESULTS</b>67 strains of streptomycin, isoniazid or rifampicin resistant Mycobacteria tuberculosis were found in routine drug resistance test, with the percentages of 80.5% (54/67), 58.2% (39/67) respectively. PCR-SSCP showed that out of 67 drug-resistant strains, 66(98.5%) of rpsL, 47(70.1%) of rpoB and 42(62.7%) of KatG appeared abnormal.</p><p><b>CONCLUSION</b>Most of the resistant strains appeared gene mutation. The mution rates were higher than the results from routine drug resistance test.</p>